Production of 6-Hydroxynicotinic Acid from Nicotinic Acid by Resting Cells ofPseudomonas fluorescensTN5
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چکیده
منابع مشابه
Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.
The enzymes of a Bacillus species that hydroxylate nicotinic acid to 6-hydroxynicotinic acid and 6-hydroxynicotinic acid to 2,6-dihydroxynicotinic acid were purified and characterized. The purified enzymes contained approximately two molecules of flavine and eight molecules of iron per molecule of enzyme. The enzymes were large (molecular weight, 400,000 to 450,000) and appeared to consist of s...
متن کاملOxidation of nicotinic acid by a Bacillus species: regulation of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.
The first two enzymes employed by a Bacillus species for the dissimilation of nicotinic acid are coordinately induced. The inducer of the enzymes appears to be 6-hydroxynicotinic acid, the product of the first enzyme in the pathways. Synthesis of the enzymes is repressed by glucose when ammonium is present in the medium, but not when nicotinic acid is the sole nitrogen source. The possible sign...
متن کاملNicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid.
Previous reports from this laboratory have described the isolation of 6-hydroxynicotinic acid and 6-0x0-1 ,4,5,6-tetrahydronicotinic acid from the reaction media of a clostridial species grown on nicotinic acid (1). Bn enzyme that catalyzes the reversible oxidation of nicotinic acid to HNA’ has been purified from extracts of this microorganism (2). Since fortified crude extract of this Clostrid...
متن کاملOxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid.
Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms. (18)O is incorporated into the products from H(2) (18)O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required...
متن کاملProduction of vanillic acid from vanillin by resting cells of Serratia marcescens.
Resting-cell suspensions of Serratia marcescens were able to convert, quantitatively, 0.3% vanillin to vanillic acid. The vanillic acid-producing activity reached a maximum after 28 h of incubation with 0.01% vanillin as an inducer.
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1994
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.58.665